Plant Protoplast Agglutination by Lectins

Plant protoplast agglutination by artificial carbohydrate antigens.

The existence of beta-lectins on protoplast surfaces is confirmed by the agglutination of protoplasts by those Yariv antigens that have sugar specificities which also interact with isolated beta-lectins. Agglutination by beta-maltosyl but not by beta-D-mannosyl Yariv antigens is used to identify some of the structural features required of the ligand for beta-lectin binding. Inhibition of agglut...

Agglutination of Sindbis virus and of cells infected with Sindbis virus by plant lectins.

We have examined the agglutination of Sindbis virus and of chick and hamster cells infected with Sindbis virus by two of the plant lectins, concanavalin A and Ricinus communis agglutinin. Both lectins agglutinate the virus by binding to the polysaccharide chains of the envelope glycoproteins. Both chick and hamster cells exhibit increased agglutination by the lectins after infection by Sindbis ...

Lectins agglutination test as an epidemiological marker for Neisseria gonorrhoeae.

Cell hybridization and cell agglutination. I. Enhancement of cell hybridization by lectins.

A great increase in hybridization frequency of cultured rodent cells was obtained when conventional cell fusion using 50% polyethylene glycol (PEG) was combined with a cell agglutination produced by plant lectins. The rate of appearance of hybrid colonies was found to be correlated with the extent of cell agglutination by lectin, as well as with cell fusion induced by subsequent PEG treatment. ...

Role of Lectins in Plant - Microorganism Interactions

The influence of rhizosphere/rhizoplane culture conditions on the ability of various rhizobia to bind soybean seed lectin (SBL) was examined. Eleven strains of the soybean symbiont, Rhizobium japonicun4 and six strains of various heterologous Rhizobium species were cultured in root exudate of soybean ( Glycine max IL.I Merr.) and in association with roots of soybean seedlings which were growing...